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101.
Omics approaches on fresh‐cut lettuce reveal global molecular responses to sodium hypochlorite and peracetic acid treatment 下载免费PDF全文
Loretta Daddiego Linda Bianco Cristina Capodicasa Fabrizio Carbone Claudia Dalmastri Lorenza Daroda Antonella Del Fiore Patrizia De Rossi Mariasole Di Carli Marcello Donini Loredana Lopez Alessio Mengoni Patrizia Paganin Gaetano Perrotta Annamaria Bevivino 《Journal of the science of food and agriculture》2018,98(2):737-750
102.
Lucio Boschetti Matteo Ottavian Pierantonio Facco Massimiliano Barolo Lorenzo Serva Stefania Balzan Enrico Novelli 《Meat science》2013
The use of near-infrared spectroscopy (NIRS) is proposed in this study for the characterization of the quality parameters of a smoked and dry-cured meat product known as Bauernspeck (originally from Northern Italy), as well as of some technological traits of the pork carcass used for its manufacturing. In particular, NIRS is shown to successfully estimate several key quality parameters (including water activity, moisture, dry matter, ash and protein content), suggesting its suitability for real time application in replacement of expensive and time consuming chemical analysis. Furthermore, a correlative approach based on canonical correlation analysis was used to investigate the spectral regions that are mostly correlated to the characteristics of interest. The identification of these regions, which can be linked to the absorbance of the main functional chemical groups, is intended to provide a better understanding of the chemical structure of the substrate under investigation. 相似文献
103.
Comparison of nine common coffee extraction methods: instrumental and sensory analysis 总被引:3,自引:0,他引:3
Alexia N. Gloess Barbara Schönbächler Babette Klopprogge Lucio D`Ambrosio Karin Chatelain Annette Bongartz André Strittmatter Markus Rast Chahan Yeretzian 《European Food Research and Technology》2013,236(4):607-627
The preparation of a cup of coffee may vary between countries, cultures and individuals. Here, an analysis of nine different extraction methods is presented regarding analytical and sensory aspects for four espressi and five lunghi. This comprised espresso and lungo from a semi-automatic coffee machine, espresso and lungo from a fully automatic coffee machine, espresso from a single-serve capsule system, mocha made with a percolator, lungo prepared with French Press extraction, filter coffee and lungo extracted with a Bayreuth coffee machine. Analytical measurements included headspace analysis with HS SPME GC/MS, acidity (pH), titratable acidity, content of fatty acids, total solids, refractive indices (expressed in °Brix), caffeine and chlorogenic acids content with HPLC. Sensory analysis included visual, aroma, flavor and textural attributes as well as aftersensation. The technical differences in the extraction methods led to a higher concentration of the respective quantities in the espressi than in the lunghi. Regarding the contents per cup of coffee, the lunghi generally had a higher content than the espressi. The extraction efficiency of the respective compounds was mainly driven by their solubility in water. A higher amount of water, as in the extraction of a lungo, generally led to higher extraction efficiency. Comparing analytical data with sensory profiles, the following positive correlations were found total solids ? texture/body, headspace intensity ? aroma intensity, concentrations of caffeine/chlorogenic acids ? bitterness and astringency. 相似文献
104.
Demétrius Paiva Arçari Juliana Carvalho Santos Alessandra Gambero Lucio Fábio Caldas Ferraz Marcelo Lima Ribeiro 《Molecular nutrition & food research》2013,57(10):1882-1885
The aim of this study was to evaluate the effects of yerba maté (YM) extract on the phosphatidylinositol 3‐kinase (PI3K)‐AKT signaling pathway in vivo. The mice were introduced to either standard‐ or high‐fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 g/kg. After treatment, glucose blood level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA levels using the PI3K‐AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility‐shift assay. Our data demonstrated that yerba maté extract significantly decreased the final body weight, glucose blood levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba maté treatment, the levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba maté on the PI3K‐AKT pathway, which contributed to the observed improvement in hepatic insulin signaling. 相似文献
105.
Andrea Martinelli Massimo Calì Lucio D'Ilario Iolanda Francolini Antonella Piozzi 《应用聚合物科学杂志》2012,123(5):2697-2705
In the first part of this article, we reported the crystalline memory effect on the nonisothermal crystallization of poly(L ‐lactide). The experiments were carried out by using polymer single crystals growth from dilute solution as standard starting material. In this article (Part II), we have analyzed in detail the effect of the melting condition on the overall crystallization kinetics by applying the Nakamura‐Avrami model to DSC results. The absence or the low concentration of foreign infusible heterogeneous nuclei in our system allowed us to exalt the self‐nuclei role in polymer crystallization, to follow their concentration decrease during the melting process and to find the limiting melting temperature for their disappearance. Below such a temperature, a stable equilibrium number of self‐nuclei was observed, probably deriving from ordered structures, persisting in the melt, and originated from the single crystals thickening process during the polymer dynamic melting in the DSC. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012 相似文献
106.
Noam Ben-Eliezer Eddy Solomon Elad Harel Nava Nevo Lucio Frydman 《Magma (New York, N.Y.)》2012,25(6):433-442
Object
An approach has been recently introduced for acquiring arbitrary 2D NMR spectra or images in a single scan, based on the use of frequency-swept RF pulses for the sequential excitation and acquisition of the spins response. This spatiotemporal-encoding (SPEN) approach enables a unique, voxel-by-voxel refocusing of all frequency shifts in the sample, for all instants throughout the data acquisition. The present study investigates the use of this full-refocusing aspect of SPEN-based imaging in the multi-shot MRI of objects, subject to sizable field inhomogeneities that complicate conventional imaging approaches.Materials and methods
2D MRI experiments were performed at 7 T on phantoms and on mice in vivo, focusing on imaging in proximity to metallic objects. Fully refocused SPEN-based spin echo imaging sequences were implemented, using both Cartesian and back-projection trajectories, and compared with k-space encoded spin echo imaging schemes collected on identical samples under equal bandwidths and acquisition timing conditions.Results
In all cases assayed, the fully refocused spatiotemporally encoded experiments evidenced a ca. 50?% reduction in signal dephasing in the proximity of the metal, as compared to analogous results stemming from the k-space encoded spin echo counterparts.Conclusion
The results in this study suggest that SPEN-based acquisition schemes carry the potential to overcome strong field inhomogeneities, of the kind that currently preclude high-field, high-resolution tissue characterizations in the neighborhood of metallic implants. 相似文献107.
Selective hydrogenation of benzene to cyclohexene using a suspended Ru catalyst in a mechanically agitated tetraphase reactor 总被引:4,自引:0,他引:4
The reactivity on unsupported Ru based catalyst in benzene selective hydrogenation to cyclohexene has been studied. The reaction has been carried out in a tetraphase slurry reactor at 423 K, at 5 MPa, in the presence of two liquid phases: benzene and an aqueous solution of ZnSO4 0.6 mol l−1. A detailed study of the influence of the transport phenomena on the reactivity of the catalyst has been carried out. No correlation has been found between the characteristic numbers of Weeler–Weisz and of Carberry mass transport at external liquid/solid interface or into the catalyst pores for both benzene and hydrogen and the selectivity of the catalyst. The main features of the catalysts are the strong dependence between the catalysts preparation procedure and their activity and selectivity. In particular the influence of the alkaline or the earth alkaline hydroxide, employed in the precipitation of the Ru precursor, on the selectivity, has been studied. Hydrogen chemisorption measurements indicate that the amount of weakly adsorbed hydrogen depends on the nature of the base employed in the precipitation step. 相似文献
108.
Giulio Verna Marina Liso Elisabetta Cavalcanti Giusy Bianco Veronica Di Sarno Angelo Santino Pietro Campiglia Marcello Chieppa 《International journal of molecular sciences》2021,22(15)
Dendritic cells (DCs) can be divided by lineage into myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs). They both are present in mucosal tissues and regulate the immune response by secreting chemokines and cytokines. Inflammatory bowel diseases (IBDs) are characterized by a leaky intestinal barrier and the consequent translocation of bacterial lipopolysaccharide (LPS) to the basolateral side. This results in DCs activation, but the response of pDCs is still poorly characterized. In the present study, we compared mDCs and pDCs responses to LPS administration. We present a broad panel of DCs secreted factors, including cytokines, chemokines, and growth factors. Our recent studies demonstrated the anti-inflammatory effects of quercetin administration, but to date, there is no evidence about quercetin’s effects on pDCs. The results of the present study demonstrate that pDCs can respond to LPS and that quercetin exposure modulates soluble factors release through the same molecular pathway used by mDCs (Slpi, Hmox1, and AP-1). 相似文献
109.
Rossana Di Martino Maria Jos Sisalli Rossana Sirabella Salvatore Della Notte Domenica Borzacchiello Antonio Feliciello Lucio Annunziato Antonella Scorziello 《International journal of molecular sciences》2021,22(15)
The exact mechanism underlying selective dopaminergic neurodegeneration is not completely understood. The complex interplay among toxic alpha-synuclein aggregates, oxidative stress, altered intracellular Ca2+-homeostasis, mitochondrial dysfunction and disruption of mitochondrial integrity is considered among the pathogenic mechanisms leading to dopaminergic neuronal loss. We herein investigated the molecular mechanisms leading to mitochondrial dysfunction and its relationship with activation of the neuroinflammatory process occurring in Parkinson’s disease. To address these issues, experiments were performed in vitro and in vivo in mice carrying the human mutation of α-synuclein A53T under the prion murine promoter. In these models, the expression and activity of NCX isoforms, a family of important transporters regulating ionic homeostasis in mammalian cells working in a bidirectional way, were evaluated in neurons and glial cells. Mitochondrial function was monitored with confocal microscopy and fluorescent dyes to measure mitochondrial calcium content and mitochondrial membrane potential. Parallel experiments were performed in 4 and 16-month-old A53T-α-synuclein Tg mice to correlate the functional data obtained in vitro with mitochondrial dysfunction and neuroinflammation through biochemical analysis. The results obtained demonstrated: 1. in A53T mice mitochondrial dysfunction occurs early in midbrain and later in striatum; 2. mitochondrial dysfunction occurring in the midbrain is mediated by the impairment of NCX3 protein expression in neurons and astrocytes; 3. mitochondrial dysfunction occurring early in midbrain triggers neuroinflammation later into the striatum, thus contributing to PD progression during mice aging. 相似文献
110.
Benedicta Normanna Limata Lucio Gialanella Nicola De Cesare Antonio D’Onofrio G. Gyurky Mario Romano Detlef Rogalla Michele Russo Filippo Terrasi 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》2008,266(10):2117-2121
A pure 7Be beam with an energy E = 1-8 MeV is available for nuclear and applied physics at the 3 MV Pelletron tandem accelerator CIRCE in Caserta. The beam is produced using an offline technique. Typical analyzed beam intensities are about 2 ppA, using cathodes with an activity of the order of 200 MBq. The 7Be implantation has been used for both fundamental nuclear physics and applied physics. In particular, different metals have been implanted with 7Be in order to study the influence of the chemical composition and of the number of quasi-free electrons of the host material on the 7Be half-life. In the field of applied physics, the 7Be implantation turns out to be very interesting for wear measurement. In fact, in this case 7Be is used as a depth-sensitive tracer. The continuous detection of the sample activity during the wear allows a high sensitivity measurement of wearing speed. The 7Be beam production at CIRCE, the implantation procedure and the results obtained from the 7Be half-life measurements and the wear characterization of implanted steel samples are described. 相似文献